Coagulation

I.     Course Prefix/Number: MLT 115

       Course Name: Coagulation

       Credits: 1 (0.5 lecture; 1 lab)

II.    Prerequisite

MLT 105, 106, 111, and 112, with a minimum grade of C in each course.

III.   Course (Catalog) Description

Course includes classroom lecture and laboratory course in coagulation. Content includes pathways and mechanisms which make it possible for blood to clot or remain fluid.

IV.   Learning Objectives

Upon successful completion of this course the student will be able to:

  1. Define hemostasis and coagulation and describe the waterfall (cascade) theory of coagulation including the role of each factor in the intrinsic and extrinsic pathways.
  2. Compare the coagulation factors; list their sources of production, function, their absence or presence in absorbed plasma and aged serum; include each factor’s position in the coagulation pathway.
  3. List the Vitamin K dependent factors.
  4. Summarize specimen collection techniques for coagulation studies including sources of error.
  5. Interpret the effects of anticoagulant therapy on the patient, including HIT and HITT.
  6. Differentiate the principle, procedure and results of each test used for following anticoagulant therapy.
  7. Perform PT and APTT by the fibrometer method according to laboratory protocol.
  8. Perform the test for the D-dimer; include the principle of the test.
  9. Compare the theory, procedure, sources of error and identifying factors measured by a thrombin clotting time test and a fibrinogen assay.
  10. Perform the thrombin clotting time and fibrinogen assay according to laboratory protocol.
  11. Differentiate thrombosis risk factors for APTT for LA, Protein C, Protein S, APC resistance, antithrombin assay, Factor V Leiden, Prothrombin 20210A, Lupus anticoagulant screening, and antiphospholipid antibody testing.
  12. Interpret PT and PTT mixing studies; include the purpose and principle of each.
  13. Outline the procedure for correlating PT and PTT results to diagnose factor deficiencies.
  14. Interpret results and perform the following procedures:  thrombocyte counts, bleeding times, activated clotting time test, clot retraction and clot lysis, and Rumpel-Leede tourniquet test; include the principles, clinical purposes and normal values for each test.
  15. Compare fibrinolysis and DIC by summarizing diagnosing tests and results.
  16. Define the following terms:  disseminated intravascular coagulation (DIC), petechiae, ecchymosis, purpura, fibrin monomer, fibrinolysis, FDP, thrombocyte, thrombopenia, thrombocytosis, thrombopathy, plasminogen,  vasoconstriction, platelet adhesiveness, platelet aggregation,  diapedesis,  hematoma, thrombosis, and embolism.
  17. Summarize the principle and procedures involved in factor assays.
  18. Address platelet structure and function; include normal and abnormal platelet architecture and platelet factors.
  19. Compare the principles for platelet function tests--platelet aggregation and platelet adhesiveness.
  20. Correlate quantitative and qualitative deficiencies of platelets with disease states.

V.    Academic Integrity and Student Conduct

Students and employees at Oakton Community College are required to demonstrate academic integrity and follow Oakton's Code of Academic Conduct. This code prohibits:

• cheating,
• plagiarism (turning in work not written by you, or lacking proper citation),
• falsification and fabrication (lying or distorting the truth),
• helping others to cheat,
• unauthorized changes on official documents,
• pretending to be someone else or having someone else pretend to be you,
• making or accepting bribes, special favors, or threats, and
• any other behavior that violates academic integrity.

There are serious consequences to violations of the academic integrity policy. Oakton's policies and procedures provide students a fair hearing if a complaint is made against you. If you are found to have violated the policy, the minimum penalty is failure on the assignment and, a disciplinary record will be established and kept on file in the office of the Vice President for Student Affairs for a period of 3 years.

Please review the Code of Academic Conduct and the Code of Student Conduct, both located online at
www.oakton.edu/studentlife/student-handbook.pdf

VI.   Sequence of Topics

  1. Hemostasis and coagulation principles (activated zymogens and the waterfall theory)
  2. Specimen collection and anticoagulants
  3. Coagulation factors, vasoconstriction, and platelets roles in coagulation
  4. Principles and general consideration, advantages and disadvantages and primary use of the following tests:
    1. Platelet counts (review)
    2. Bleeding times: template
    3. Activated Clotting Time Test
    4. Prothrombin time
    5. Activated partial thromboplastin times
    6. D-dimer test
    7. Thrombin Clotting Time Test
    8. Fibrinogen assays
    9. Thrombosis testing:
      1. APTT for LA
      2. Protein C
      3. Protein S
      4. APC resistance
      5. Antithrombin assay
      6. Factor V Leiden
      7. Prothrombin 20210A
      8. Lupus anticoagulant screening
      9. Antiphospholipid antibody testing
    10. PT and APTT mixing studies
    11. Factor assays
    12. Platelet aggregation
    13. Platelet adhesiveness
    14. Clot retraction
    15. Factor Xa assay

VII.  Methods of Instruction

Classroom lectures, discussion of case studies, slide tape series, reading assignments, and laboratory demonstrations will be used to present theory and techniques.

Student laboratory practice sessions analyzing biological specimens will be used to develop student skill and accuracy in coagulation.


Course may be taught as face-to-face, hybrid or online course.

VIII. Course Practices Required

  1. Course is taught face-to-face, hybrid or online course.
  2. Participate in all sessions and activities. 
  3. Attend all lectures and laboratory sessions.
  4. Complete assigned readings, study questions, worksheets, and any other specific assignments for class.
  5. Complete lab assignment each week.
  6. Complete all assigned Media Lab modules.

IX.   Instructional Materials

Note: Current textbook information for each course and section is available on Oakton's Schedule of Classes.

X.    Methods of Evaluating Student Progress

  1. The final grade will be based on the total number of points earned on exams, homework assignments, a comprehensive final exam, laboratory work, attendance and class participation.
  2. All study questions must be turned in to pass this course even if no points will be earned. Points will be deducted for incomplete/late assignments as follows:
    Incomplete (one answer missing)   – 1 point
    Incomplete (more than two answers missing)  – 2 points
    Late (handed in next class session) – 2 point
    Late (handed in 2 classes later) – 3 points
    Late (handed in >2 classes later) – 5 points
  3. Your final grade will be calculated as follows:
    Exam 1 100 points
    Exam 2 100 points
    Quizzes 20 points
    Final Comprehensive Exam  120 points
    Study Questions 30 points
    Case Study Homework 10 points
    Laboratory worksheets 30 points
    Laboratory Practical Exam 40 points
    Total Points Possible 450 points
  4. The final grade will be based on the total points earned as follows:
    Points Earned  Grade Equivalent
    414 – 450 A
    378 – 413 B
    342 – 377 C
    315 – 341 D
    Below 315 F

XI.   Other Course Information

Students must notify the instructor if they will be late or absent. Attendance and class participation are included in grading.  Points will be deducted for excessive absences and late arrivals to class as they disrupt class for your classmates.

Students must successfully complete both the lecture and laboratory components of the course (this means earning a 76% or above in each component).

No makeup quizzes or exams will be given unless the instructor agrees (to give the exam) prior to the class meeting.  Students will be permitted to make up only one exam.  The exam must be completed in a timely manner for full credit.  Instances where the exam is not completed before the next class session may result in a reduction of points.  Students that do not complete an exam in the regularly scheduled time slot will still be required to complete it, but may receive little or no credit depending on the circumstances.

Students are responsible for material covered in class, even if missed.  If absent, it is advised that students contact someone in class for this information prior to the next class session.  Your instructor is willing to assist you in reviewing any material you do not understand.  Please initiate the contact for such help.

All assignments are due at the beginning of class on the date specified; late papers will have points deducted. If you are absent on the date an assignment is due you must make arrangements with the instructor to be allowed to turn it in for credit. All assignments must be completed in order to earn a grade for the class.

Failure to comply with established laboratory waste disposal policies will result in loss of laboratory points.

Established laboratory safety protocol must be followed while in the laboratory including: no eating or drinking in the lab, removal of personal protective equipment and washing of hands before leaving lab, and others discussed in class.

Proper clothing must be worn to all laboratory sessions which includes no shorts or open toe shoes.  Long hair must also be tied back.   If shorts or open toe shoes are worn to class, you will not be allowed to participate in the lab session.  You will be asked to leave and points will be deducted for an absence.  The lab will need to be made up in open lab.

No special projects or term papers will be accepted in lieu of class assignments, written exams, or active participation in class.

College policies regarding Academic Dishonesty, Student Procedure for Appeal of a Final Grade, and Code of Student Conduct, are described in the catalogue.

Oakton Community College recognizes the broad diversity of religious beliefs of its constituencies.  The college has embraced a practice of shared responsibility in the event a religious observance interferes with class work or assignments.  Students who inform instructors in advance of an intended absence for a major religious observance will not be penalized.  The instructor will make reasonable accommodation for students, which may include providing a make-up test, altering assignment dates, permitting a student to attend another section of the same course for a class period or similar remedies.  Instructors are not responsible for teaching material againInstructors should inform students of this practice at the beginning of the semester so that arrangements can be made accordingly.

If you have a documented learning, psychological, or physical disability you may be entitled to reasonable academic accommodations or services.  To request accommodations or services, contact the Access and Disability Resource Center at the Des Plaines or Skokie campus.  All students are expected to fulfill essential course requirements.  The College will not waive any essential skill or requirement of a course or degree program.

Health Status Change Policy Statement – Any change in health status of currently enrolled students, resulting in the inability to meet the course/program objectives and standards as outlined in the Essential Skills requirement policy will require documentation and medical approval for the student to return to clinical, theory, and lab-which require lifting without restrictions.

  1. Any change in health status must be reported to the Chair of the department.  Examples may include but are not limited to, back injury, pregnancy, infection such as shingles, fractures, etc.
  2. Students must provide documentation of care by an Illinois licensed physician or an Illinois certified nurse practitioner and submit a medical release without restrictions before returning to clinical/class.
  3. Releases from physicians or nurse practitioners must state that the student “can return to the laboratory and clinical facility without any work restrictions.”

A change in health status must be reported to the Chair of the department.  Failure to submit a medical release or information regarding a change in health status within 30 days is grounds for immediate dismissal from a Health Career Program.



If you have a documented learning, psychological, or physical disability you may be entitled to reasonable academic accommodations or services. To request accommodations or services, contact the Access and Disability Resource Center at the Des Plaines or Skokie campus. All students are expected to fulfill essential course requirements. The College will not waive any essential skill or requirement of a course or degree program.

Oakton Community College is committed to maintaining a campus environment emphasizing the dignity and worth of all members of the community, and complies with all federal and state Title IX requirements.

Resources and support for
  • pregnancy-related and parenting accommodations; and
  • victims of sexual misconduct
can be found at www.oakton.edu/title9/.

Resources and support for LGBTQ+ students can be found at www.oakton.edu/lgbtq.