Medical Microbiology

I.     Course Prefix/Number: MLT 225

       Course Name: Medical Microbiology

       Credits: 4 (3 lecture; 3 lab)

II.    Prerequisite

MLT 215 and BIO 251, with a minimum grade of C in each course.

III.   Course (Catalog) Description

Course provides an in-depth study of medical microbiology, including mycology and parasitology.  The course covers terminology and methods used to isolate and identify pathogens.

IV.   Learning Objectives

Upon successful completion of this course the student will be able to:

  1. Summarize basic concepts of medical microbiology dealing with immunology, pathogenicity, disease transmission and control, sterilization and disinfection techniques, and the use of the specialized equipment, methods for stains and basic nutritional requirements of microorganisms.
  2. List common anaerobes, and identify special anaerobic collection and culturing techniques, anaerobic cultural characteristics, biochemical reactions and morphological characteristic. Summarize the effects of anaerobic toxins.
  3. Apply information found in Objective 2.
  4. Identify medically important gram positive and negative cocci and bacilli, using cultural and morphological characteristics, biochemical reactions, and immunological procedures.
  5. List normal and abnormal flora of specific body sites.
  6. Outline cultural procedures for specimens from specific body sites.
  7. Discuss antibiotic sensitivity testing include theory and procedures.
  8. Summarize major classifications of viruses and special aspects of laboratory diagnostic procedures.
  9. Outline basic mycological procedures for identification of common, medically important fungi.
  10. Outline specimen collection procedures and laboratory methods for diagnosis of parasitic infections.
  11. Identify parasites.
  12. Compare parasitic life cycles.
  13. Correlate parasites with disease states and clinical signs.
  14. Summarize the principle and procedures of quality control in the medical microbiology lab.
  15. Orally present Parasitology report on selected parasite according to established criteria.
  16. Demonstrate teaching ability through peer teaching and group discussion.

V.    Academic Integrity and Student Conduct

Students and employees at Oakton Community College are required to demonstrate academic integrity and follow Oakton's Code of Academic Conduct. This code prohibits:

• cheating,
• plagiarism (turning in work not written by you, or lacking proper citation),
• falsification and fabrication (lying or distorting the truth),
• helping others to cheat,
• unauthorized changes on official documents,
• pretending to be someone else or having someone else pretend to be you,
• making or accepting bribes, special favors, or threats, and
• any other behavior that violates academic integrity.

There are serious consequences to violations of the academic integrity policy. Oakton's policies and procedures provide students a fair hearing if a complaint is made against you. If you are found to have violated the policy, the minimum penalty is failure on the assignment and, a disciplinary record will be established and kept on file in the office of the Vice President for Student Affairs for a period of 3 years.

Please review the Code of Academic Conduct and the Code of Student Conduct, both located online at
www.oakton.edu/studentlife/student-handbook.pdf

VI.   Sequence of Topics

  1. Basic Concepts
  2. Gram Positive Cocci
  3. Gram Negative Cocci
  4. Gram Negative Rods
  5. Non-fermenters
  6. Gram Positive Rods
  7. Anaerobes
  8. Additional Pathogens and Viruses
  9. Mycobacteria
  10. Antimicrobial Sensitivity Testing
  11. Normal Flora, Set-Up and Follow Up Procedures, Quality Control and Rapid Methods
  12. Parasitology
  13. Mycology

VII.  Methods of Instruction

Method of presentation includes lectures, films, video cassettes and kodachrome slides.  Laboratory sessions will provide the student with the opportunity to develop skills in microbiological techniques and experience in identification of microorganisms.


Course may be taught as face-to-face, hybrid or online course.

VIII. Course Practices Required

  1. Course taught face-to-face, hybrid or online course.
  2. Reading assignments
  3. Writing assignments
  4. Oral presentation
  5. Computer use
  6. Lab practices
  7. Class participation
  8. Complete assigned Media Lab modules

Attendance at additional open lab sessions (Mondays, 12:00-3:00 pm) may be necessary to satisfactorily complete all laboratory assignments.

IX.   Instructional Materials

Note: Current textbook information for each course and section is available on Oakton's Schedule of Classes.

X.    Methods of Evaluating Student Progress

  1. The final grade will be based on the total number of points earned as follows:
    Micro Exam I 100 pts.
    Micro Exam II 100 pts.
    Micro Exam III 100 pts.
    Micro Exam IV 100 pts.
    Comprehensive Final Exam    100 pts.
    Laboratory 250 pts.
    Parasitology Report 50 pts.

    Total Points Possible 800 pts.
  2. A total of 250 points may be earned in laboratory work; therefore the lab component is 25% of the final grade. All lab work must be completed to pass the course; any absences must be made up in open lab sessions.
    Point distribution for Microbiology Lab is as follows:
    Staining unknowns (11 at 5 pts each)    55 points
    Bacterial unknowns (9 at 10 pts each) 90 points
    Parasitology unknowns 10 points
    Lab Practical 30 points
    Lab worksheets 15 points
    Quizzes 50 points
  3. The Parasitology Report consists of a written and oral report on an assigned parasite. The report should include a description of the organism, its life cycle, stages of development and detailed methods of identification. Be sure to include descriptions or pictures of each stage and possible problems in identification. At least three sources must be utilized in the report preparation; one of them should be the CDC website.
  4. The final grade will be based on total points earned:
    Points earned   Grade Equivalent
    736 - 800 A
    672 - 735 B
    608 - 671 C
    560 - 607 D
    below 560 F

XI.   Other Course Information

Students must notify the instructor if they will be late or absent. Attendance and class participation are included in grading.  Points will be deducted for excessive absences and late arrivals to class as they disrupt class for your classmates.

Students must successfully complete both the lecture and laboratory components of the course (this means earning a 76% or above in each component).

No make up quizzes or exams will be given unless the instructor agrees (to give the exam) prior to the class meeting.  Students will be permitted to make up only one exam.  The exam must be completed in a timely manner for full credit.  Instances where the exam is not completed before the next class session may result in a reduction of points.  Students that do not complete an exam in the regularly scheduled time slot will still be required to complete it, but may receive little or no credit depending on the circumstances.

Students are responsible for material covered in class, even if missed.  If absent, it is advised that students contact someone in class for this information prior to the next class session.  Your instructor is willing to assist you in reviewing any material you do not understand.  Please initiate the contact for such help.

All assignments are due at the beginning of class on the date specified; late papers will have points deducted. If you are absent on the date an assignment is due you must make arrangements with the instructor to be allowed to turn it in for credit. All assignments must be completed in order to earn a grade for the class.

Failure to comply with established laboratory waste disposal policies will result in loss of laboratory points.

Established laboratory safety protocol must be followed while in the laboratory including: no eating or drinking in the lab, removal of personal protective equipment and washing of hands before leaving lab, and others discussed in class.

Proper clothing must be worn to all laboratory sessions which includes no shorts or open toe shoes.  Long hair must also be tied back.   If shorts or open toe shoes are worn to class, you will not be allowed to participate in the lab session.  You will be asked to leave and points will be deducted for an absence.  The lab will need to be made up in open lab.

No special projects or term papers will be accepted in lieu of class assignments, written exams, or active participation in class.

College policies regarding Academic Dishonesty, Student Procedure for Appeal of a Final Grade, and Code of Student Conduct, are described in the catalogue.

Oakton Community College recognizes the broad diversity of religious beliefs of its constituencies.  The college has embraced a practice of shared responsibility in the event a religious observance interferes with class work or assignments.  Students who inform instructors in advance of an intended absence for a major religious observance will not be penalized.  The instructor will make reasonable accommodation for students, which may include providing a make-up test, altering assignment dates, permitting a student to attend another section of the same course for a class period or similar remedies.  Instructors are not responsible for teaching material againInstructors should inform students of this practice at the beginning of the semester so that arrangements can be made accordingly.

If you have a documented learning, psychological, or physical disability you may be entitled to reasonable academic accommodations or services.  To request accommodations or services, contact the Access and Disability Resource Center at the Des Plaines or Skokie campus.  All students are expected to fulfill essential course requirements.  The College will not waive any essential skill or requirement of a course or degree program.

Health Status Change Policy Statement – Any change in health status of currently enrolled students, resulting in the inability to meet the course/program objectives and standards as outlined in the Essential Skills requirement policy will require documentation and medical approval for the student to return to clinical, theory, and lab-which require lifting without restrictions.

  1. Any change in health status must be reported to the Chair of the department.  Examples may include but are not limited to, back injury, pregnancy, infection such as shingles, fractures, etc.
  2. Students must provide documentation of care by an Illinois licensed physician or an Illinois certified nurse practitioner and submit a medical release without restrictions before returning to clinical/class.
  3. Releases from physicians or nurse practitioners must state that the student “can return to the laboratory and clinical facility without any work restrictions.”

A change in health status must be reported to the Chair of the department.  Failure to submit a medical release or information regarding a change in health status within 30 days is grounds for immediate dismissal from a Health Career Program.



If you have a documented learning, psychological, or physical disability you may be entitled to reasonable academic accommodations or services. To request accommodations or services, contact the Access and Disability Resource Center at the Des Plaines or Skokie campus. All students are expected to fulfill essential course requirements. The College will not waive any essential skill or requirement of a course or degree program.

Oakton Community College is committed to maintaining a campus environment emphasizing the dignity and worth of all members of the community, and complies with all federal and state Title IX requirements.

Resources and support for
  • pregnancy-related and parenting accommodations; and
  • victims of sexual misconduct
can be found at www.oakton.edu/title9/.

Resources and support for LGBTQ+ students can be found at www.oakton.edu/lgbtq.