Applications in Laboratory Practice

I.     Course Prefix/Number: MLT 235

       Course Name: Applications in Laboratory Practice

       Credits: 3 (3 lecture; 0 lab)

II.    Prerequisite

MLT 210 and MLT 225, with a minimum grade of C in each course.

III.   Course (Catalog) Description

Course deals with presentation and utilization of basic mathematics concepts as applied in the medical laboratory. Focus is on laboratory quality control and governmental regulations. Review of all major areas also included.

IV.   Learning Objectives

Upon successful completion of this course the student will be able to:

  1. Solve problems in metric conversion, temperature conversion, concentrations of solutions, quality control, hematological manipulations.
  2. Summarize basic principles of quality assurance in the laboratory.
  3. Address the relationship between the laboratory and various governmental and professional agencies.
  4. Compare basic principles of operation for selected laboratory instruments.
  5. Correlate knowledge gained in the MLT program with ability to apply to entry level competencies.
  6. Demonstrate problem solving/troubleshooting ability through participation in higher taxonomic domain exercises.
  7. Orally present Instrumentation report according to established criteria.
  8. Demonstrate teaching ability through peer teaching and group discussion.

V.    Academic Integrity and Student Conduct

Students and employees at Oakton Community College are required to demonstrate academic integrity and follow Oakton's Code of Academic Conduct. This code prohibits:

• cheating,
• plagiarism (turning in work not written by you, or lacking proper citation),
• falsification and fabrication (lying or distorting the truth),
• helping others to cheat,
• unauthorized changes on official documents,
• pretending to be someone else or having someone else pretend to be you,
• making or accepting bribes, special favors, or threats, and
• any other behavior that violates academic integrity.

There are serious consequences to violations of the academic integrity policy. Oakton's policies and procedures provide students a fair hearing if a complaint is made against you. If you are found to have violated the policy, the minimum penalty is failure on the assignment and, a disciplinary record will be established and kept on file in the office of the Vice President for Student Affairs for a period of 3 years.

Please review the Code of Academic Conduct and the Code of Student Conduct, both located online at

VI.   Sequence of Topics

  1. Math Review
    1. Metric System
    2. Temperature conversion
    3. Solutions
    4. Molarity and Normality
    5. Dilutions
    6. Neutralizations
  2. Quality control
    1. Calculation of standard deviation
    2. Confidence limits
    3. Quality control charting
    4. Definitions of terms
      1. Mean
      2. Median
      3. Mode
      4. Coefficient of variation
      5. Trend
      6. Shift
  3. Hematological mathematics
    1. Manipulation of hemocytometer dimensions
    2. Computation of cell counts
    3. Computation of red cell indices
    4. Correlation of red cell indices to red cell morphology
    5. Correction for NRBC's
    6. Computation of absolute vs. relative counts
    7. Computation for hemoglobin
    8. Computation fro reticulocytes
  4. Professional Organizations and Government Agencies
    1. ASCP
    2. ASCLS
    3. CAP
    4. HFAP
    5. Joint Commission
    6. OSHA
    7. CDC
    8. CLSI
    9. HIPAA
    10. COLA
    11. CLIA 88
    12. CMS
    13. FDA
    14. NAACLS
  5. Laboratory instrumentation
  6. Review of laboratory areas
    1. Selected case studies
    2. Review of study questions

VII.  Methods of Instruction

Methods of presentation include lectures, films, videocassettes and kodachrome slides.

Course may be taught as face-to-face, hybrid or online course.

VIII. Course Practices Required

  1. Course is taught face-to-face, hybrid or online course.   
  2. Completion of assigned math problems
  3. Completion of assigned clinical study questions
  4. Reading assignments
  5. Writing assignments
  6. Oral presentation
  7. Computer use
  8. Class participation
  9. Complete all assigned Media Lab modules

IX.   Instructional Materials

Note: Current textbook information for each course and section is available on Oakton's Schedule of Classes.

X.    Methods of Evaluating Student Progress

  1. The final grade will be based on the total number of points earned on the quizzes, homework assignments, and attendance. Homework assignments include math practice sheets, study questions and a written report and oral presentation on a particular piece of laboratory equipment (which we will decide upon in class).
  2. The student must pass each math unit exam to pass the course – this means a grade of at least 75 (out of 100 points) on each math test. Make up exams will be given in cases where a score lower than 75 is earned.
  3. Points will be deducted for late assignments.
  4. The final grade will be based on the total number of points earned as follows:
    Math Quiz 1 100 points
    Math Quiz 2 100 points
    Math Quiz 3 100 points
    Math Quiz 4 100 points
    Math Homework 40 points
    Instrumentation Paper and Presentation    50 points
    Study Questions/Case Studies 100 points
    Quiz on Missions 10 points
    Case study assignment 20 points
    Hematology Slide Quiz 60 points
    Urinalysis Slide Quiz 60 points
    Microbiology Slide Quiz 110 points

    Total Points Possible 850 points
  5. The final grade will be based on total points earned:
    Points earned   Grade Equivalent
    782 – 850 A
    714 – 781 B
    646 – 713 C
    595 – 645 D
    Below 595 F

XI.   Other Course Information

Students must notify the instructor if they will be late or absent. Attendance and class participation are included in grading.  Points will be deducted for excessive absences and late arrivals to class as they disrupt class for your classmates.

Students must successfully complete both the lecture and laboratory components of the course (this means earning a 76% or above in each component).

No makeup quizzes or exams will be given unless the instructor agrees (to give the exam) prior to the class meeting.  Students will be permitted to make up only one exam.  The exam must be completed in a timely manner for full credit.  Instances where the exam is not completed before the next class session may result in a reduction of points.  Students that do not complete an exam in the regularly scheduled time slot will still be required to complete it, but may receive little or no credit depending on the circumstances.

Students are responsible for material covered in class, even if missed.  If absent, it is advised that students contact someone in class for this information prior to the next class session.  Your instructor is willing to assist you in reviewing any material you do not understand.  Please initiate the contact for such help.

All assignments are due at the beginning of class on the date specified; late papers will have points deducted. If you are absent on the date an assignment is due you must make arrangements with the instructor to be allowed to turn it in for credit. All assignments must be completed in order to earn a grade for the class.

Failure to comply with established laboratory waste disposal policies will result in loss of laboratory points.

Established laboratory safety protocol must be followed while in the laboratory including: no eating or drinking in the lab, removal of personal protective equipment and washing of hands before leaving lab, and others discussed in class.

Proper clothing must be worn to all laboratory sessions which includes no shorts or open toe shoes.  Long hair must also be tied back.   If shorts or open toe shoes are worn to class, you will not be allowed to participate in the lab session.  You will be asked to leave and points will be deducted for an absence.  The lab will need to be made up in open lab.

No special projects or term papers will be accepted in lieu of class assignments, written exams, or active participation in class.

College policies regarding Academic Dishonesty, Student Procedure for Appeal of a Final Grade, and Code of Student Conduct, are described in the catalogue.

Oakton Community College recognizes the broad diversity of religious beliefs of its constituencies.  The college has embraced a practice of shared responsibility in the event a religious observance interferes with class work or assignments.  Students who inform instructors in advance of an intended absence for a major religious observance will not be penalized.  The instructor will make reasonable accommodation for students, which may include providing a make-up test, altering assignment dates, permitting a student to attend another section of the same course for a class period or similar remedies.  Instructors are not responsible for teaching material againInstructors should inform students of this practice at the beginning of the semester so that arrangements can be made accordingly.

If you have a documented learning, psychological, or physical disability you may be entitled to reasonable academic accommodations or services.  To request accommodations or services, contact the Access and Disability Resource Center at the Des Plaines or Skokie campus.  All students are expected to fulfill essential course requirements.  The College will not waive any essential skill or requirement of a course or degree program.

Health Status Change Policy Statement – Any change in health status of currently enrolled students, resulting in the inability to meet the course/program objectives and standards as outlined in the Essential Skills requirement policy will require documentation and medical approval for the student to return to clinical, theory, and lab-which require lifting without restrictions.

  1. Any change in health status must be reported to the Chair of the department.  Examples may include but are not limited to, back injury, pregnancy, infection such as shingles, fractures, etc.
  2. Students must provide documentation of care by an Illinois licensed physician or an Illinois certified nurse practitioner and submit a medical release without restrictions before returning to clinical/class.
  3. Releases from physicians or nurse practitioners must state that the student “can return to the laboratory and clinical facility without any work restrictions.”

A change in health status must be reported to the Chair of the department.  Failure to submit a medical release or information regarding a change in health status within 30 days is grounds for immediate dismissal from a Health Career Program.

If you have a documented learning, psychological, or physical disability you may be entitled to reasonable academic accommodations or services. To request accommodations or services, contact the Access and Disability Resource Center at the Des Plaines or Skokie campus. All students are expected to fulfill essential course requirements. The College will not waive any essential skill or requirement of a course or degree program.

Oakton Community College is committed to maintaining a campus environment emphasizing the dignity and worth of all members of the community, and complies with all federal and state Title IX requirements.

Resources and support for
  • pregnancy-related and parenting accommodations; and
  • victims of sexual misconduct
can be found at

Resources and support for LGBTQ+ students can be found at

Electronic video and/or audio recording is not permitted during class unless the student obtains written permission from the instructor. In cases where recordings are allowed, such content is restricted to personal use only. Any distribution of such recordings is strictly prohibited. Personal use is defined as use by an individual student for the purpose of studying or completing course assignments.

For students who have been approved for audio and/or video recording of lectures and other classroom activities as a reasonable accommodation by Oakton’s Access Disabilities Resource Center (ADRC), applicable federal law requires instructors to permit those recordings. Such recordings are also limited to personal use. Any distribution of such recordings is strictly prohibited.

Violation of this policy will result in disciplinary action through the Code of Student Conduct.